C (3-6 months) Cre/loxP system 2009年 理化学研究所横浜免疫アレルギー研究所の古関 明彦先生と千葉大学岩間先生研究室との共同研究で作製した。 R1 ES (Hybrid of two 129 substrains (129X1/SvJ and 129S1/SV-+<p>+<Tyr-c>Kitl<Sl-J>/+)。 Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc] Homozygote x Homozygote [or Crossing to C57BL/6JJcl] B6.129-Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)> B6.129-Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)> 2009年 理化学研究所横浜免疫アレルギー研究所の古関 明彦先生と千葉大学岩間先生研究室との共同研究で作製した。R1 ES (Hybrid of two 129 substrains (129X1/SvJ and 129S1/SV-+<p>+<Tyr-c>Kitl<Sl-J>/+)。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> ROSA26遺伝子座にloxP-STOP-loxP-FLAG-Bmi1-FRT-IRES-EGFP-FRTを挿入したノックインマウス。造血細胞特異的にBmi1を発現させると造血幹細胞活性が増殖する。 岩間　厚志 Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]Homozygote x Homozygote [or Crossing to C57BL/6JJcl] In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 未定(後日指定/will be announced) 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。未定(後日指定/will be announced) R26Stop<FL>Bmi1, B6-ROSA26/Stop-Bmi1,GFP KI R26Stop<FL>Bmi1, B6-ROSA26/Stop-Bmi1,GFP KI true C57BL/6コンジェニック系統。C57BL/6NCr (日本SLC) に7世代以上の戻し交配を行う。その後，兄妹交配にて維持。交配によりY染色体はB6置換済み。 Atsushi IWAMA <a href='https://brc.riken.jp/mus/pcr05539'>Genotyping protocol -PCR-</a> R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] A loxP-flanked neo<r>-stop cassette followed by FLAG-tagged Bmil, and frt-flanked IRES-EGFP cassette, and a bovine polyA sequences were knocked in the ROSA26 locus. RBRC05539 C（3〜6か月） Developed by Atsushi Iwama, Graduate School of Medicine, Chiba University and Akihiko Koseki, RIKEN Research Center for Allergy and Immunology in 2009. The mutant mice were backcrossed to C57BL/6N. FLP/frt system Bacteriophage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, STOP, FLAG epitope tag, mouse Bmi1 cDNA, yeast FRT (flipase recombination target) sites, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Bovine PolyA signal, mouse Gt(ROSA)26Sor genomic DNA Fluorescent Proteins/lacZ System